HPLC

HPLC Method Transfer Calculator

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solvent saved.

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Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
sigma-aldrich

HPLC Method Transfer Calculator

Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Equilibration
Time(min)
17.44
3.49
4.04
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
Gradient
Conditions
Current Method
New Method
New MethodAdjust Dwell Volume
Dwell Volume(mL)
Gradient Delay(min)
0.00
0.00
Gradient
Table
TIME
% A
% B
New Time
New TimeAdjusted Flow
20
0.00
0.00
20
0.40
0.46
50
2.00
2.32
50
4.00
4.63
20
5.00
5.79
20
6.00
6.95


sigma-aldrich

HPLC Method Transfer Calculator

The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.

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