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Reflectometers, Test Strips & Visual Tests
HPLC

HPLC Method Transfer Calculator

Calculates conditions for transfer of an isocratic or gradient method from one HPLC column to another. Allows method scaling from microbore through preparative column range. Calculates time and solvent saved.

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HPLC Method Transfer Calculator

  • Isocratic method
  • gradient method
  • technical support
my test
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9
sigma-aldrich

HPLC Method Transfer Calculator

  • Isocratic method
  • gradientmethod
  • technicalsupport
my test
Current Method
New Method
New Methodadjust flow rate
Column Length(cm)
Column I.D.(mm)
Particle Size(μm)
Flow Rate(mL/min)
0.35
Injection Volume(μL)
1.4
1.4
Pressure(psi/bar)
1543.2
1332.8
Run Time (min)
6.00
6.95
Equilibration
Time(min)
17.44
3.49
4.04
Time Saved (min)
24.0
23.1
Solvent Saved (mL)
27.9
27.9

Gradient
Conditions
Current Method
New Method
New MethodAdjust Dwell Volume
Dwell Volume(mL)
Gradient Delay(min)
0.00
0.00

Gradient
Table
TIME
% A
% B
New Time
New TimeAdjusted Flow
20
0.00
0.00
20
0.40
0.46
50
2.00
2.32
50
4.00
4.63
20
5.00
5.79
20
6.00
6.95


sigma-aldrich

HPLC Method Transfer Calculator

  • Isocraticmethod
  • gradientmethod
  • technicalsupport
my test

The scaled gradient method is based on estimates of column volumes and is therefore only an approximation. More precise method scaling requires actual measured values of column volumes. Column volume is a function of particle size, particle structure, and column packing.

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  • Dia Health Care
  • Our Products
    • Chromatography >
      • HPLC Column >
        • Exmere HPLC Column
  • Broucher
  • Exmere Ltd
    • Products >
      • Exsil Mono
      • Exil 80
      • Exsil Diametric
      • Exsil 100
      • Exsil Clasico
      • Exsil Plus
      • Exsil Pure >
        • Exsil Pure C18MS
        • Exsil Pure RP
        • Exsil Polar P
      • Exsil 120 Paragon
      • Avanti
      • Avanti BDS >
        • Avanti BDS Quattro >
          • Exsil Pure 500A C18
        • Avanti BDS ALKAM
        • Avanti BDS FLUORO
      • Exsil 300 >
        • Exsil 300 Si
        • Exsil 300 C4
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        • Exsil 300 C18
        • Exsil 300 PAH
        • Exsil 300 HAAX
      • Exsil GFC >
        • Exsil GFC 3000
        • Exsil GFC 4000
        • Exsil GFC 5000
      • Exsil 500
      • Exsil 1000
      • Exsil NP
  • Customer Support
  • Review
  • Contact us