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W3.CSS
Exsil Pure 500 C18

Ultra Fast Protein and Peptide Separations 
• 10 times faster bio separations than traditional column formats 
• Ultra-high efficiency 1.5µm packing 
• Fast protein and peptide separations with conventional HPLC systems 

Optimization of Gradient Parameters for Fast Protein Analysis
Short Exsil Pure 500A columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer columns only increase run time and do not increase resolution to improve the separation.

Postulated Seperation Mechanism of Peptides and Proteins​
Proteins adsorb at the head of the column and then desorb once the critical mobile phase concentration is reached.   Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution.   Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution.  higher flow rates, and fast, modified gradients. For best results high-pressure mixing should be used with fast gradients.  ​
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  • Dia Health Care
  • Our Products
    • Chromatography >
      • HPLC Column >
        • Exmere HPLC Column
  • Broucher
  • Exmere Ltd
    • Products >
      • Exsil Mono
      • Exil 80
      • Exsil Diametric
      • Exsil 100
      • Exsil Clasico
      • Exsil Plus
      • Exsil Pure >
        • Exsil Pure C18MS
        • Exsil Pure RP
        • Exsil Polar P
      • Exsil 120 Paragon
      • Avanti
      • Avanti BDS >
        • Avanti BDS Quattro >
          • Exsil Pure 500A C18
        • Avanti BDS ALKAM
        • Avanti BDS FLUORO
      • Exsil 300 >
        • Exsil 300 Si
        • Exsil 300 C4
        • Exsil 300 C8
        • Exsil 300 C18
        • Exsil 300 PAH
        • Exsil 300 HAAX
      • Exsil GFC >
        • Exsil GFC 3000
        • Exsil GFC 4000
        • Exsil GFC 5000
      • Exsil 500
      • Exsil 1000
      • Exsil NP
  • Customer Support
  • Contact us