Exsil Pure 500 C18
Ultra Fast Protein and Peptide Separations
• 10 times faster bio separations than traditional column formats
• Ultra-high efficiency 1.5µm packing
• Fast protein and peptide separations with conventional HPLC systems
Optimization of Gradient Parameters for Fast Protein Analysis
Short Exsil Pure 500A columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer columns only increase run time and do not increase resolution to improve the separation.
Postulated Seperation Mechanism of Peptides and Proteins
Proteins adsorb at the head of the column and then desorb once the critical mobile phase concentration is reached. Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution. Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution. higher flow rates, and fast, modified gradients. For best results high-pressure mixing should be used with fast gradients.
Ultra Fast Protein and Peptide Separations
• 10 times faster bio separations than traditional column formats
• Ultra-high efficiency 1.5µm packing
• Fast protein and peptide separations with conventional HPLC systems
Optimization of Gradient Parameters for Fast Protein Analysis
Short Exsil Pure 500A columns are the perfect tool for fast reversed-phase protein separations. Under gradient conditions, longer columns only increase run time and do not increase resolution to improve the separation.
Postulated Seperation Mechanism of Peptides and Proteins
Proteins adsorb at the head of the column and then desorb once the critical mobile phase concentration is reached. Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution. Since the proteins do not interact with the full length of the packed bed, short columns are suficient for full resolution. higher flow rates, and fast, modified gradients. For best results high-pressure mixing should be used with fast gradients.
| exsil_pure_500a_c18_v1.1.pdf |