About Ghost Eliminator
Exmere Ghost-Eliminator can effectively remove impurities with low polarity and thus prevents the interference from all kinds of ghost peaks. It is installed between gradient mixer and injector, which helps remove not only the impurities in mobile phase, but impurities in mixer and pipelines as well. Unlike in-line filters which removes only solid particles but not organic pollutants, Exmere's Ghost Eliminator provides strong adsorption to weak-polar and non-polar organic impurities, without changing the composition of mobile phase, thus to purify both mobile phase and system, remove most ghost peaks and extend lifetime of column and system.
Ghost Eliminator installed between the gradient mixer and the sampler (injector)
Where is Ghost Peak from ?
Ghost Eliminator uses a specifically optimized stationary phase and improved hardware. Ghost Eliminator removes impurities in the mobile phase with stronger retention.
Stable Baseline :
When the initial proportion of aqueous phase is high (generally more than 95%), using conventional Ghost columns can remove impurities effectively. But some ghost peaks may still occur when the proportion of mobile phase has a drastic change in a few minutes or the baseline has large fluctuation. By improving the overall design of the Ghost Eliminator, the mobile phase is fully mixed before entering the analytical column, greatly reducing the baseline fluctuation and drift in the initial phase of the gradient program.
Ordering Information :
Packing : Ghost Eliminator
Dim : 50x4.6 mm
Part no: ghost.s0546
Do & Don't
Q1: For different samples and gradient conditions, should the Ghost-Eliminator column be removed or changed?
Ans : Not necessary. But it needs to be removed only for special circumstances like changing of peak position or ion-pair solvents mobile phase.
Q2 : When gradient elution changed to isocratic, should the Ghost-Eliminator column be removed?
Ans : No need to take the Ghost-Eliminator column if it did not affect the separation, as the elution of mobile phase stays same under isocratic condition. But impurities in mobile phase shall be taken into consideration.
Q3 : In gradient system, Ghost-Eliminator column increases the mixed dwell volume. Will this affect the separation?
Ans : The packing volume of a 4.6×50mm column is ~400 µL and the column is installed before the injector, which would cause little influence on the analysis. If it does, connect Ghost-Eliminator column to the water phase path before the mixer or switching valve.
Q4 : Any requirements for the connecting of Ghost-Eliminator column?
Ans : No special requirements for the connection. Common PEEK tube and connectors for HPLC system is recommended.
Q5: What’s the lifetime of GHOST ELIMINATOR column?
Ans : Not necessary. But it needs to be removed only for special circumstances like changing of peak position or ion-pair solvents mobile phase. The lifetime of GHOST ELIMINATOR column is related to the analysis conditions, brand of the solvents and purity of the mobile phase. If the mobile phase composition (such as water/methanol) is simple, and GHOST ELIMINATOR column is carefully used, the lifetime of the GHOST ELIMINATOR column is over one year and the number of injections is around 3000 times. Replacement of the GHOST ELIMINATOR column is recommended once the trapping effect begins to deteriorate
Q6: What’s the washing procedure and how frequently we have to wash the column?
Ans : GHOST ELIMINATOR column doesn’t need special washing as the adsorption of impurities is irreversible.
Q7 : Is GHOST ELIMINATOR column compatible with ion-pair reagent mobile phases?
Whether ion pair mobile phase can be used should be determined by testing a new GHOST ELIMINATOR column as the sorbent in the GHOST ELIMINATOR column will absorb ion pair reagent.
Q8 :Can GHOST ELIMINATOR column be used for different types of mobile phases (such as potassium phosphate, sodium phosphate, ammonium acetate, TFA, Formic acid, etc.?
Yes, GHOST ELIMINATOR column can be used for different type of mobile phase except the mobile phase containing ammonium ion. Other reagents such as potassium phosphate, sodium phosphate, TFA, formic acid, etc. can be used for the column.